Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Tuberculosis (TB) is the biggest cause of human mortality from an infectious disease. The only vaccine currently available, bacille Calmette-Guérin (BCG), demonstrates some protection against disseminated disease in childhood but very variable efficacy against pulmonary disease in adults. A greater understanding of protective host immune responses is required in order to aid the development of improved vaccines. Tissue-resident memory T cells (TRM) are a recently-identified subset of T cells which may represent an important component of protective immunity to TB. Here, we demonstrate that intradermal BCG vaccination induces a population of antigen-specific CD4+ T cells within the lung parenchyma which persist for >12 months post-vaccination. Comprehensive flow cytometric analysis reveals this population is phenotypically and functionally heterogeneous, and shares characteristics with lung vascular and splenic CD4+ T cells. This underlines the importance of utilising the intravascular staining technique for definitive identification of tissue-resident T cells, and also suggests that these anatomically distinct cellular subsets are not necessarily permanently resident within a particular tissue compartment but can migrate between compartments. This lung parenchymal population merits further investigation as a critical component of a protective immune response against Mycobacterium tuberculosis (M. tb).

Original publication

DOI

10.1016/j.vaccine.2018.07.035

Type

Journal article

Journal

Vaccine

Publication Date

05/09/2018

Volume

36

Pages

5625 - 5635

Keywords

BCG, Immunogenicity, T cell, Tissue-resident, Tuberculosis, Vaccine, Adjuvants, Immunologic, Animals, BCG Vaccine, CD4-Positive T-Lymphocytes, Female, Immunogenicity, Vaccine, Immunologic Memory, Interferon-gamma, Lung, Mice, Mice, Inbred BALB C, Parenchymal Tissue, Tuberculosis, Pulmonary