Alterations in plasma protein N-glycosylation after caloric restriction and bariatric surgery.
Štambuk T., Kifer D., Greto VL., Dempster NJ., Cvetko A., Gillies RS., Tomlinson JW., Sgromo B., Mineo C., Shaul PW., Lauc G., Lingvay I., Geremia A., Arancibia-Cárcamo CV.
BACKGROUND: Protein glycosylation is an enzymatic process known to reflect an individual's physiologic state and changes thereof. The impact of metabolic interventions on plasma protein N-glycosylation has only been sparsely investigated. OBJECTIVE: To examine alterations in plasma protein N-glycosylation following changes in caloric intake and bariatric surgery. SETTING: University of Texas Southwestern Medical Center, US and Oxford University Hospitals, UK. METHODS: This study included 2 independent patient cohorts that recruited 10 and 37 individuals with obesity undergoing a period of caloric restriction followed by bariatric surgery. In both cohorts, clinical data were collated, and the composition of plasma protein N-glycome was analyzed chromatographically. Linear mixed models adjusting for age, sex, and multiple testing (false discovery rate