The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues. Here, we present a protocol for harvesting mouse SCN by vibrating microtome for diurnal transcriptome analysis. We describe steps for mouse entrainment, isolation of the SCN, tissue preparation, slicing with a vibratome, and handling of the harvested SCN for RNA extraction. This protocol can also be used for harvesting other mammalian brain regions for genomic studies.
Journal article
2023-12-15T00:00:00+00:00
4
Genetics, Model Organisms, Molecular Biology, Neuroscience, Mice, Animals, Circadian Rhythm, Suprachiasmatic Nucleus, Circadian Clocks, Gene Expression Profiling, Histological Techniques, Mammals