Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Duchenne muscular dystrophy (DMD) is an X-linked recessive muscle wasting disease caused by the absence of a muscle cytoskeletal protein, dystrophin. Utrophin is the autosomal homologue of dystrophin. We previously demonstrated that overexpression of utrophin in the muscles of dystrophin-null transgenic mice completely prevented the phenotype arising from dystrophin deficiency. Two independently regulated promoters control utrophin expression and the upstream promoter (promoter A) is synaptically regulated in muscle. In this study, we have investigated basal regulation and myogenic induction of promoter A. Interactions between Ap2 and Sp1 and their cognate DNA motifs are critical for basal transcription from the minimal promoter region. During differentiation of C2C12 myoblasts in vitro, a 2-fold increase in A-utrophin mRNA level was observed. Expression of a reporter gene, whose transcription was driven by a 1.3 kb promoter A fragment, paralleled expression of the endogenous transcript. Myogenic induction mapped to a conserved upstream muscle-specific E-box, which was shown to bind myogenic regulatory factors, transactivating the promoter up to 18-fold in transient assays. This study provides a basis for further understanding the regulatory mechanisms that control utrophin expression in muscle and may facilitate the development of reagents to effect therapeutic up-regulation of utrophin in DMD.

Original publication

DOI

10.1093/nar/29.23.4843

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

01/12/2001

Volume

29

Pages

4843 - 4850

Keywords

5' Flanking Region, Animals, Base Sequence, Cell Differentiation, Cell Line, Conserved Sequence, Cytoskeletal Proteins, DNA-Binding Proteins, Humans, Membrane Proteins, Mice, Molecular Sequence Data, Muscle, Skeletal, Muscular Dystrophy, Duchenne, Myogenic Regulatory Factors, Promoter Regions, Genetic, RNA, Messenger, Sp1 Transcription Factor, Transcription Factor AP-2, Transcription Factors, Transcriptional Activation, Up-Regulation, Utrophin